Kjeldahl’s method:
Kjeldahl method purpose :
This method can be used for Estimation of Nitrogen in a given Compound.
Kjeldahl's method procedure:
In Kjeldahl method, Nitrogen is converted into Ammonia. Ammonia produced is directly proportional to Nitrogen.During this conversion, CuSO4 used as Catalyst
17 gram of Ammonia = 14 gram of Nitrogen
Ammonia is quantitatively calculated by using Acid with known concentration
(N*V) Acid = (N * V) Base
LIMITATIONS of Kjeldahl's Method:
Kjeldahl method can't detect Nitrogen in,
(i) organic compounds containing nitrogen in the ring such as pyridine, quinoline, etc.
(ii) Organic compounds containing nitrogen (–NO2) and diazo (–N = N–) groups.
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